Bio-Rad EXQuest Spot Cutter - Manual (Page 61)

PDQuest User Guide
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The quantities of saturated spots can be estimated, reported as the value -3.0, or
reported in some other way that you specify. If you opt for the latter, click the Other
button and enter the value that should be reported.
If a spot is unmatched in one or more members of a MatchSet, it can be estimated as
the value -1.0 or as a value that you enter in the text box next to the Other button.
For each spot, you can include the SSP number, DSN (if designated), replicate group
ID, and/or MrpI (Peak) value if calculated by selecting the appropriate checkboxes.
You can export annotations in all categories or a specified category. If you select
Specified, select the category from the pulldown list.
You can export all analysis sets or a specified analysis set. To specify an analysis set,
select one from the pulldown list.
Under Spots to Export, specify the spots whose data should be exported. You can
choose all the spots in the MatchSet, those spots that are matched in every member of
the MatchSet, or the spots in a particular analysis set. The spot count automatically
adjusts to reflect the total number of spots selected.
Under Output Options, you can include headers for each column in the report. Select
how you want to sort the spot data: by SSP number, DSN, or gel/replicate group.
Select the field separators you want to use to separate columns of data in your
spreadsheets: commas or tabs for Excel.
Your data can be exported as a file or to the clipboard. If you choose the file option
button, clicking Done opens the Export MatchSet Data dialog box, in which you can
enter a name and export location for the file. The file type automatically defaults to an
.xls spreadsheet.
If you choose the clipboard option, clicking Done saves your data to the system
clipboard.
If you have created replicate groups for the MatchSet, you can export the averaged
data for each group. Under Spot Data by Group, select the data to export (Master
position, average quantity, coefficient of variation, standard Gaussian size, average
normalized quantity, normalized coefficient of variation, and/or the counts in the
member gels).