Bio-Rad EXQuest Spot Cutter - Manual (Page 329)

PDQuest User Guide
10-6
Fig. 10-2. Mass Spec Score Overlay dialog.
In the dialog box, indicate the instrument you want to report scores for by selecting
MALDI or MS/MS. Note that if there is no data for an instrument, no spot overlays
will be shown.
Next, select the type of score by selecting Joint Probability or MOWSE.
Enter the threshold value for the score in the Threshold field. Spots above this
threshold can be considered confident hits; spots below this threshold are not.
Click the Format buttons next to Above Threshold, Below Threshold, and No Hits to
select the appearance of the different overlays.
No Hits are spots with no hits or with an unknown protein identification. This means
that the mass spec tried and failed to recognize the sequence.
10.4 Protein Probe
If you have imported mass spec data for spots using Import MassLynx Results, you
can launch the Protein Probe browser from within PDQuest to review the protein
information for those spots.
Note: Protein Probe must be installed on the same computer as PDQuest to use this
tool.