Bio-Rad EXQuest Spot Cutter - Manual (Page 197)

PDQuest User Guide
6-6
In the stack tool window, all available gels are listed in the Select gels to display field.
To select an image to display, click on a gel name. The name is highlighted with an
arrow and the image appears in the window.
Click on another gel name to display that image.
Using the Playback controls you can step forward or backward through the images in
the stack tool. First, select some or all of the gel names using standard
SFT +click or
CNTRL +click key commands. When multiple names are selected, the back and
forward Step arrow buttons become active. Click the arrow buttons to scroll through
the list of selected gels. Your current gel selection is highlighted with an arrow in the
gel display window.
Alternatively, click the Auto checkbox next to the arrow buttons to begin
automatically scrolling through the list. You can adjust the auto-scroll speed using the
Slow-Fast slider. To stop auto-scrolling, deselect the checkbox.
You can rearrange the order of the images with the image order arrows.
Buttons for various viewing tools are included in the stack tool. These commands will
change the display of all the images in the stacker at once (e.g., zooming in on one
image will magnify the same relative area in all the images).
With the image flagging tools, it is possible to compare spots across gels and flag
spots of interest manually. There are two new commands: Save flagged spot set and
Clear spot flags. Place your cursor on the spot you want to flag and use C
NTRL +click
to flag the spot. The cursor becomes an orange circle that shows which spot will be
flagged (marked for stacking). Use Clear spot flags to delete any spots you added. Or
use S
FT + C NTRL +click to unflag spots.
If the spot is flagged with a red cross it needs to be added to the Master gel. If the spot
is flagged with a green cross that means the spot has been matched to the Master gel.
If images flicker back and forth between the gel members in two windows the
flickering cursor becomes a green or red X. The Save flag spot set button prompts you
to add flagged spots to the Master. If you click Yes, a pop-up box prompts you to
name and describe the flagged spot set. The flags will then stay in the MatchSet when
you close the Image Stack tool.
To display the spot crosshairs on the images in a MatchSet check the Show Crosshairs
box. Flags are treated the same as crosshairs. If you restart the program (reopen the
MatchSet) the flags are still there.