Bio-Rad EXQuest Spot Cutter User Manual

Page 6

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Contents

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5.2. Selecting the Master ...................................................................................... 5-9

5.3. Matching Spots ............................................................................................. 5-10

5.4. Landmarking Spots ....................................................................................... 5-14

5.5. Automated Matching ................................................................................... 5-17

5.6. Cybergels ...................................................................................................... 5-23

5.7. Adding Spots to the Master .......................................................................... 5-26

5.8. Edit Match Submenu .................................................................................... 5-29

5.9. Matching Summary ...................................................................................... 5-37

6.

Analysis Tools .................................................................... 6-1

6.1. Spot Review Tool ........................................................................................... 6-1

6.2. Image Stack Tool ............................................................................................ 6-5

6.3. Scatter Plot Tool ............................................................................................. 6-7

6.4. Replicate Groups ............................................................................................ 6-9

6.5. Group Consensus .......................................................................................... 6-13

6.6. Sample Database ........................................................................................... 6-17

6.7. Normalization ............................................................................................... 6-23

6.8. MrpI Data ...................................................................................................... 6-29

6.9. Standard Spot Numbers ................................................................................ 6-32

6.10. Database Spot Numbers .............................................................................. 6-34

7.

Analysis Sets and Annotations ........................................ 7-1

7.1. Analysis Sets ................................................................................................... 7-1

7.2. Annotation Tool ............................................................................................ 7-22

7.3. Browsing Annotations .................................................................................. 7-33

7.4. Creating Annotations from Analysis Sets ..................................................... 7-34

7.5. Printing Annotations ..................................................................................... 7-36

7.6. Transferring Annotations .............................................................................. 7-37

8.

Basic Excision Tool ........................................................... 8-1

8.1. Spot Cutter Setup ............................................................................................ 8-2

8.2. Cutting Spots .................................................................................................. 8-8

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