Bio-Rad EXQuest Spot Cutter - Manual (Page 325)

PDQuest User Guide
10-2
Step 1
In the dialog box, the MatchSet name is listed next to MatchSet. Select the cut list you
want to analyze from the Cut List pulldown list. If you have performed more than one
cut run on this cut list, select the number of the run from the Cut Run Serial Number
pulldown list.
Next, select the plate used for the cut run using the Microtiter Plate pulldown list. If
more than one plate was used for the run, select the first plate you want to process.
Step 2
Select the appropriate Micromass instrument using the MALDI or MS/MS option
button.
Step 3
The Required Project Name is the name of the MassLynx project that must be created
for this cut list. Open MassLynx and create a project with this name. See MassLynx
documentation for information on how to create project files.
Note: We recommend that you create a MassLynx project file from an existing project
or a default template, to ensure that the default parameter files are stored in the
correct location inside the project.
When you have created a project, you can click the Find button (if the path to the
project directory is mapped correctly as described above) or navigate to it yourself by
clicking on the MassLynx Project pulldown button.
Step 4
In this step, you specify the mass spec parameters to be used for processing the
proteins. See Micromass documentation for descriptions of the parameters and their
functions.
Note: You must map the Micromass paths as described above to specify the parameters.
Select the acquisition parameters using the Acquisition Parameters pulldown list.
Select the processing program you want to use using the Process With pulldown list.