Bio-Rad EXQuest Spot Cutter - Manual (Page 282)

Basic Excision Tool
8-17
Fig. 8-12. Confirmation image; note that the first cut made incorrectly.
When you have confirmed that the cuts are being made in the correct locations, click
Begin/Resume to proceed with the rest of the cuts. Otherwise, you can abort the run.
Ending the Cut Run
Click Pause to pause the cut run. You will be prompted to confirm the pause. Note
that the cutter will pause only after the most recent cut has been deposited in a well.
Any remaining cut requests will remain selected in the image. At this point, you can
click Begin/Resume to complete the remainder of the cuts, or abort the run.
When all cuts are complete, the cutting tip will return to home position and you will
receive an alert message on the screen. At this point, you can visually inspect the cut
holes for visible spots, or take a UV image of the MTP and confirm the cuts.
8.2.f Recutting Spots
You can specify re-cuts by clicking the Recuts button below the image window, or
when using the UV mode, follow the on-screen directions for imaging the MTP.
Cut 1,A01 has been
missed.
Cut 1,A02 was made
correctly.