Bio-Rad EXQuest Spot Cutter - Manual (Page 304)

Integrated Excision Tool
9-15
been previously installed on the cutter. Make sure the grid is flat and aligned with
the stage edges. Click Ready to proceed. You may also skip this step, return to the
previous dialog, cancel the action, or ask for help.
Step 3: The fourth dialog prompts you to place the coomassie stained PVDF membrane
on the mat. Click Ready to proceed with cutting, Back to return to the previous
screen, or Cancel to cancel the action.
At any point in the procedure you can move back to repeat a step.
Note: Calibration creates several parameter and image files in the Install.prm directory
in the Discovery Series directory on your hard drive. These files should not be
moved, deleted or altered, or calibration will be affected.
When calibration is complete, you can proceed with normal use of the spot cutter.
Once the cutter is calibrated, you do not need to repeat the procedure during normal
day-to-day use. You must recalibrate if:
• The instrument is moved.
• You install or remove the lens filter after calibration.
9.2.c Cutting Spots
After you have focused the camera and calibrated the spot cutter, you are ready to
begin cutting spots. The control panel is designed to guide you through the spot
cutting procedure.