Bio-Rad EXQuest Spot Cutter - Manual (Page 311)

PDQuest User Guide
9-22
Note: For UV exposures, there will be a 15-second delay while the lamps heat up
before the exposure begins.
The camera will take a single image of the gel or membrane on the platform, then a
background image to adjust for image background. When the exposure is complete,
the image appears in the window.
To adjust the brightness and contrast of the image, open the Transform
window by clicking on the button to the right of the Image window.You can
also use the Transform button on the main toolbar.
Step 2 Positioning the Data Area Box
You will be prompted to position the green data area box that surrounds the camera
cutter image.
Fig. 9-11. Position the data area box.
If the orientation of the image is incorrect, use the flip and/or rotate buttons to fix the
orientation so that it matches the MatchSet image. Next, position your cursor over the
box and drag it so that it defines the same area as the left-hand (MatchSet) image.
Click OK. To reposition the data area box at a later time, click the Position Data Box
button and drag the box.
Step 3 Perform Alignment
In step three click Auto to have PDQuest automatically align the image. If you wish
to manually align the image, click Manual.