Bio-Rad EXQuest Spot Cutter - Manual (Page 280)

Basic Excision Tool
8-15
8.2.c Step 3. Set Cutter Options
Before beginning a cut run using the Basic Excision Tool, you must select the cutter
options for that run.
If Spin When Cutting is selected, the cutting tip will spin as it cuts.
Spin When Ejecting will cause the cutting tip to spin as it ejects the cut into a
microplate well.
Cut Longer will cause the cutting tip to hold in the “down” position longer when
making a cut.
Double Eject will cause the cutting tip to emit two blasts of air when ejecting a cut
into a microtiter plate well.
The Gel Defaults button automatically selects the most useful options when cutting
gels. The PVDF Defaults button automatically selects the most useful options when
cutting PVDF membranes.
8.2.d Step 4. Set Wash Options
Select the wash stations to use after each cut by clicking on one or more checkboxes.
8.2.e Performing a Cut Run
After you have identified all the cuts you want to make and selected the appropriate
options, you are ready to begin the cut run. If you are using a fluorescent spot cutter,
close the door of the enclosure before proceeding.
Click the Begin/Resume button. You will be prompted to place a microtiter plate in
the plate holder.
As each cut is being made, it will be highlighted in yellow on the screen. After each
cut has been made, the cut circle appears in white on the screen.Cuts that have not yet
been made are circled in green.