Bio-Rad EXQuest Spot Cutter - Manual (Page 137)

PDQuest User Guide
4-16
Note: You can add and remove spots in the MatchSet and/or the scanset. Note that, after
a MatchSet has been created, subsequent changes in the scanset are not
automatically transferred to the MatchSet, and visa versa.
Make sure that both the Filtered image and Gaussian image are loaded before adding
and removing spots. In a MatchSet, use Interchange Images (F11, main toolbar) to
toggle between the two images.
The tools for adding and removing spots are located on the Spots menu and Edit Spots
toolbar.
Adding Spots
If a spot in the Filtered image has not been added to the Gaussian image,
select Add Spot from the Spots menu or Edit Spots toolbar, then click the
spot in the Filtered image. The spot will be Gaussian modeled and added to
the Gaussian image.
Note: It is also possible to add spots on Raw 2-D scans when the Filtered and the
Gaussian images are loaded.