Bio-Rad EXQuest Spot Cutter - Manual (Page 296)

Integrated Excision Tool
9-7
gels, the rows are spots. You can use this table to make cut requests or modify the
auto-selection of cuts.
Under Table Options, click Show (Quality) to display the spot quality in the table
cells in parentheses following the spot quantity.
Click Show Ratio to display a column in the table showing the quantity tolerance ratio
across all gels for each spot. This value is used in conjunction with the Tolerance
Ratio setting, described above.
Show All Gels will show columns for all the gels in the MatchSet. If this is
unselected, only those gels with cut requests will be listed in the table.
Image Options
Display Cuts marks each cut request with a red box in the MatchSet gel(s), and also
shows the actual cut request circles. These circles indicate the region of the spot that
will be cut. Note that if the Multiple Cuts/Spot checkbox is selected, multiple circles
may appear on some or all spots.
Auto-zoom on cut will automatically magnify each spot you select in the table.
Active Gels Only displays only those gels listed in the table. Note that if the Show All
Gels checkbox is not selected, only those gels with cut requests are listed in the table,
and consequently the MatchSet will reconfigure to show only those gels.
Under Cut options, the Multiple Cuts/Spot option automatically calculates the size of
each spot that has a cut request and, if the spot is sufficiently large, specifies multiple
cuts of that spot. Enter a maximum number of cuts per spot in the Max Cuts/Spot
field. The multiple cut circles will be displayed in the MatchSet. To customize the
way multiple cuts are made for a spot, click the Specify Cuts button. This allows you
to redraw a cut box around a large spot.
9.1.c Step 3. Specify Cuts
In this step, you review the cut selection in the table and make changes as appropriate.
Note: Only those gels with cut requests will be listed in the table, unless the Show All
Gels checkbox is selected.