Bio-Rad EXQuest Spot Cutter - Manual (Page 182)

Matchsets
5-31
Otherwise, if auto-matching has missed a spot that should have been matched, you
can landmark it to improve the alignment and modeling of the Master, or you can
manually match it.
Note: Manual matching will not change the modeling of the Master, and therefore will
not facilitate additional matching.
Select Manual Match from the Match toolbar or Match > Edit Matches
submenu and click on the spot in the Master, then click on the same spot in
all the member images where that spot appears. The spot will be marked
with a purple box to indicate that it has been manually matched.
To unmatch a manual match, select Manual Unmatch from the toolbar or
submenu and click on the manually matched spot in a member image.
Unmatched Spots
Choose Mark Unmatched Spots (Shift+F8) to circle spots you have been unable to
match between gels in red. These are also displayed using the Show Symbols (F7)
command on the Match submenu.
Choose Cancel Unmatched Spots to eliminate unmatched spots from the MatchSet.
To undo this command, select Restore Unmatched Spots.
Use the Zoom Box tool to study each unmatched spot in detail in each gel.
If a spot was missed during detection, you need to add it to the Gaussian image, then
match. If a spot was not included in the Master, you need to add it to the Master. If an
unmatched spot is not significant you can cancel it.
Partial Matches
Partial matches are spots in the MatchSet Master that are not matched in one or more
member images. Review these spots to see if they were undetected, mismatched,
cancelled, or simply not present.
Select Mark Partial Matches from the Match > Edit Matches submenu and click in the
Master. The partial matches will be circled in the Master.