3 matching spots, Matching spots -10 – Bio-Rad EXQuest Spot Cutter User Manual

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PDQuest User Guide

5-10

With your Master created, you are ready to begin matching and editing spots in the
MatchSet (see the following chapter).

Displaying MatchSets

A MatchSet is displayed in a single window, with the MatchSet members in different
subwindows. The MatchSet Master initially appears in the upper left subwindow and
has the name Master.

As with scansets, you can configure the subwindows in a MatchSet using the
Configure Subwindows command (section 3.2, Configuring Subwindows), and assign
images to different subwindows using the Assign command (section 3.3, Assigning
and Interchanging Images).

The Gaussian image and Filtered image (if included) of each member gel are both
loaded in the same subwindow. To toggle between the two images, use Interchange
Images (F11) or Interchange All Images (Shift+F11). The member name and type of
image are listed at the bottom of each subwindow.

5.3 Matching Spots

After you have created a MatchSet and selected the Master template, you are ready to
begin matching spots and adding unique spots in the gels to the Master.

In a MatchSet, spot data from the member gels are included in the Master via
matching. All the significant spots in your MatchSet should be matched with spots in
the Master.

There are two types of automated matching: Automated Matching and Classic
Matching.

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