Bio-Rad EXQuest Spot Cutter - Manual (Page 207)

PDQuest User Guide
6-16
cycle through the spots in the active group. The Review Tool displays a quantity
graph of the selected spot. The bars in the graph represent the spot’s quantity in each
member of the group where it appears.
To select a different group to review, select a different cell in the Consensus Summary
table.
To select a spot in a gel, click the button labeled Select Spot in Gel then click on a
spot in one of the gel images. The spot is automatically selected and the group to
which it belongs is highlighted in the Consensus Summary table.
To see spot number in the gel, check the box labeled, “Display SSPs”. When this box
is checked, the spot number appears below the marker in the gel.
6.5.b Achieving Consensus
The following actions are used to make changes to the replicate group to achieve
consensus across all members in a group.
Automatic
Auto Add – Allows you to automatically add the selected spot to all the gels.
Clicking Auto Add will add the spot marker to the gels where it does not appear. A
red circle indicates where the spot marker is added.
Auto Remove – removes the selected spot from the gels.
Auto Cancel – Cancels the spot in the group. This does not remove the spot from the
list, but it does remove it from your analysis.
Manual
The Spot edit actions allow you to manually add or remove, cancel or restore,
combine or un-combine spots on the images in the replicate group. Click update
under the Consensus Summary table for the changes to take effect.
When you have finished making your changes to the consensus groups, click Update
under the Consensus Summary table.