Bio-Rad EXQuest Spot Cutter - Manual (Page 143)

PDQuest User Guide
4-22
To reposition a spot boundary, make sure the Contour, Freehand, or Select tool is
active, then position your cursor in the middle of the spot boundary. The cursor will
change to a multidirectional arrow; simply drag the boundary to a new location. The
spot will also appear relocated in the Gaussian image.
Copying Spot Boundaries Between Images
To copy a spot boundary between Filtered images, first select it (the
boundary will change to green), then select Copy to Clipboard from the
submenu or toolbar.
Open or select the image you want to copy to, and select Paste from
Clipboard from the submenu or toolbar. The spot boundary will appear in
the same relative position in the new image. The boundary will also appear
in the corresponding Gaussian image.
Note that the two images should have the same pixel size and approximate
dimensions. You may have to reposition the pasted boundary as described above.
4.7 Cancelling Spots
Cancelling a spot in a MatchSet or scanset preserves its location but does not include
it in matching or any other analysis. This is different than removing a spot from the
Gaussian image entirely.
Select Cancel from the Spots > More Edit Tools submenu or toolbar and click on the
spot that you want to cancel. It will disappear from the Gaussian image (it will remain
in the Filtered image, but will not be included in any analysis).
To highlight all cancelled spots in the Gaussian image, select Cancelled Spots
(Ctrl+F7) from the Spots > More Edit Tools submenu or Edit Spot toolbar.
To restore a cancelled spot, select Restore from the Spots > More Edit Tools submenu
or toolbar and click on the position of the cancelled spot. The spot will reappear.
To cancel all the spots that have not been matched in a MatchSet, select Cancel
Unmatched Spots from the Match > Edit Matches submenu and click in the MatchSet
gel.