Bio-Rad EXQuest Spot Cutter - Manual (Page 140)

Detecting and Editing Spots
4-19
Spots defined using the boundary tools are not Gaussian modeled, even though they
appear in the Gaussian image. However, their quantities can be compared with
regular, Gaussian-modeled spots.
Note: Creating a spot boundary will overwrite the results of spot detection and
Gaussian modeling. However, regular spot detection will not overwrite any spots
defined using the boundary tools.
You can open the Spot Boundary Tools toolbar from the main menu, or
select commands from the Spots > Spot Boundary Tools submenu.
Fig. 4-12. Spot boundary tools.
Spot Contour Tool
To use this tool, first magnify the spot you want to quantify in the Filtered image, then
select Spot Contour Tool from the submenu or toolbar.
Click and drag the cursor from the inside of the spot outward to change the shape of
the contour as you move over pixels of different intensity; note that the contour is
created by enclosing all pixels whose intensity is greater than or equal to the intensity
of the pixel at the cursor.
When you release the mouse button, the boundary is created. The contour should
completely surround the spot you want to quantify.