Bio-Rad EXQuest Spot Cutter - Manual (Page 144)

Detecting and Editing Spots
4-23
To restore all unmatched cancelled spots in a MatchSet, select Restore Unmatched
Spots from the Match > Edit Matches submenu and click in the MatchSet gel.
4.8 Combining Spots
Combining spots combines the quantitation of a cluster of spots into one spot. This
can be performed on Filtered images in a MatchSet or scanset.
Select Combine from the Spots > More Edit Tools submenu or Edit Spots toolbar, and
then draw a box around the cluster of spots that you want to combine in the Filtered
image. After you draw the box, click on the point in the box where you want the
combined spot to be placed. The cluster will appear as one spot in the Gaussian
image; it will still appear as individual spots in the Filtered image, but the quantitation
will be combined.
To resolve a combined spot into its original cluster, select Un-combine from the
submenu or toolbar and draw a box around the combined spot in the Filtered or
Gaussian image. The spot will be uncombined into its original multiple spots.
Combined Spots (Ctrl+F8, Spots > More Edit Tools submenu, Edit Spots toolbar)
highlights all combined spots in the MatchSet.
4.9 Saturated and Faint Spots
Saturated Spots
Spot detection may not correctly fit spots in saturated regions of the image. Therefore,
although saturated spots will appear in the Gaussian image, they are not quantified
using Gaussian modeling (their quantity will be reported as zero). You should use the
spot boundary tools (section 4.6, Spot Boundary Tools) to quantify these spots.
To highlight spots that are saturated in the image, use the Saturated Spots command
(Alt+F7, Spots > More Edit Tools submenu, Edit Spots toolbar) in any image
window. The saturated spots will appear highlighted and the number of saturated
spots will be listed at the bottom of the window.