Bio-Rad EXQuest Spot Cutter - Manual (Page 274)

Basic Excision Tool
8-9
Fig. 8-5. Light settings.
If UV light is selected, specify an exposure time in seconds in the field. Also, make
sure that the UV lamp switch on the back of the cutter box is on.
Note: For Sypro Ruby stains, an exposure time of 20-30 seconds is recommended.
When you have selected a light option, click Acquire Image.
Note: For UV exposures, there will be a 15-second delay while the lamps heat up
before the exposure begins.
The camera will take a single image of the gel or membrane on the platform, then a
background image to adjust for image background. When the exposure is complete,
the image will appear in the window.
To adjust the brightness and contrast of the image, open the Transform
window by clicking on the button to the right of the image window.