Bio-Rad EXQuest Spot Cutter - Manual (Page 276)

Basic Excision Tool
8-11
Spots selected outside the cutting area cannot be cut. Either remove the cut, or locate
it inside the cutting area.
8.2.b Step 2. Specifying Cuts
In this step, you click on spots in the image to identify which spots to cut.
Use the Zoom Box tool on the right of the Basic Excision Tool to magnify each spot.
When clicking on spots in the image, select the Snap to Peak checkbox to
automatically center the cut circle on the spot peak (calculated from the spot intensity
in the image).
To make a single cut in a spot, make sure the Multiple Cuts/Spot checkbox
is unchecked, then click the Add Cut button and click on the spot in the
image that you want to cut.
Fig. 8-7. Adding cuts.