Bio-Rad EXQuest Spot Cutter - Manual (Page 241)

PDQuest User Guide
7-14
Refer to the following table to determine the number of gels per group for you to run
a specific test.
The Wilcoxon test is only available if you are comparing classes. You must have at
least six samples per class. Follow these steps to create sample pairs in order to use
Wilcoxon test:
Step 1: Create samples in sample database (See Section 6.6, Sample Database).
Step 2: Create an attribute for these samples. Enter values for this attribute. Each
attribute-value pair defines a class. For example, an attribute could be
"treatment" with values of "treated" or "untreated". This defines two classes
"treatment=treated" and "treatment=untreated". See Section 6.6, Sample
Database, for further information on attributes.
Step 3: For the Wilcoxon test, the two classes should have same number of samples and
there should be at least 6 samples in each class.
Step 4: Pair the samples in the two classes. (See Section 6.6.d, Sample Pairs)
Step 5: Once you have created your sample pairs, go to the Analysis Set Manager. Click
create and select Statistic from the Select Set Type dialog.
Table 1: Statistic Analysis Types
Test
Replicate
Groups
Classes
Minimum
# of gels
per group
Minimum
# of
sample
pairs
Maximum
# of
samples
per group
or class
Student T Yes Yes 2 NA N/A
Mann-
Whitney Yes Yes 4 NA 20
Wilcoxon No Yes 6 6 25 (class
only)