Bio-Rad EXQuest Spot Cutter User Manual

Page 407

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PDQuest User Guide

D-8

Fig. D-7. Select Scan Resolution dialog box.

Available resolutions are listed from highest to lowest in terms of the dimensions of
the resulting pixels (in microns). Smaller pixels equal higher resolution. Each
resolution is listed with its typical use.

In general, the size of your pixels should be one-tenth the height of your smallest
object.

Some of the resolutions are asymmetrical, meaning that the resolution is higher in the
vertical dimension (i.e., the pixels in the resulting image are larger in the horizontal
dimension than in the vertical dimension). This is useful for gels with bands, where
you are more interested in resolving in the y dimension to determine the size of bands
and the spacing between them.

Specifying Your Own Resolution

If you select Oversample under More Options (see section D.7 for details), you can
specify your own resolution within the range of 43–169 microns (micrometers). With
Oversample selected, enter values directly in the fields next to X resolution and Y
resolution in the main acquisition window.

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