Bio-Rad EXQuest Spot Cutter User Manual
Page 287
PDQuest User Guide
8-22
Step 10:
Remove the plastic sheet from the top of the gel.
Step 11:
Do not move the gel. The image and spot selections that were already made still
apply to the gel as long as it does not move.
Step 12:
After removing the microtiter plate, click "OK" on the message. The "Image
Microtiter Plate" window will close and an image of the microtiter plate will
open in front of the Basic Excision Tool screen. If you need to resize the window,
close the recuts box, resize the window, make any zoom or transform changes
using the icons on the top bar above the window, then click on the "Recuts" icon
to bring the recuts box back up.
Fig. 8-19. Basic Excision Tool fluorescent mode re-cuts.
Step 13:
Enter the well numbers of the missed pick-up spots in the "Re-cuts" box on the
screen.
Step 14:
Click on "Begin/Resume" after the selections are made.