Bio-Rad EXQuest Spot Cutter - Manual (Page 533)

PDQuest User Guide
L-8
You can draw more boxes than the number of segments defined in the Calstrip
Record. However, they are automatically and permanently deactivated.
L.1.d Plot the Calibration Curve
Once you have created the sampling boxes, select Plot Calib. Curve to display the
calibration curve.
A graph will be displayed that correlates the optical density (usually between 0.00
and 2.00 O.D.) with DPMs or other specified units.
The Y axis represents the optical density (0.00–2.00) in each sampled segment, while
the X axis represents the corresponding DPM or other unit per image unit area
calculated from the calibration record data.
Fig. L-4. Example of a calibration curve.