Bio-Rad EXQuest Spot Cutter - Manual (Page 271)

PDQuest User Guide
8-6
Wash Station 1 position
Calibrate the position of Wash Station 1 on the cutting platform by following the steps
described above for Well A1. Once you have set the position of station 1, the cutter
robot will automatically “know” the positions of the remaining wash stations.
8.1.d Spot Cutter Calibration
Before you can begin excising spots, you must calibrate the spot cutter. This
procedure requires a coomassie R-250 stained PVDF membrane.
Note: Before calibration, you should focus the cutter camera and align the wash station
position. You should make sure the spot cutter camera image is properly centered
using the Crop Left Margin setting.
Note: If you are using the lens filter for UV light, make sure that it is installed BEFORE
calibration. If you add or remove the lens filter after calibration, you must
recalibrate.
Click the Calibrate Cutter button. A series of dialog boxes will guide you
through the calibration procedure. The first dialog box will prompt you to
specify whether you have installed the lens filter or not. To use in visible
mode only, calibrate with the lens filter not installed. The lens filter must be
INSTALLED if you are using the fluorescent spot cutter in fluorescent and visible
modes. The lens filter is not required if you are using the spot cutter only in visible
light mode.
Step 1: The second dialog box prompt for flat field calibration requires that you ensure
the stage is completely empty and dry. This includes the cutting mat, which must
be removed. Press Ready to proceed. If you have previously performed this step
you can skip to Step 2, although this is not recommended.