Bio-Rad EXQuest Spot Cutter - Manual (Page 165)

PDQuest User Guide
5-14
Removing MatchSet Members
To remove a MatchSet member, select the gel to be removed from the member list
and click the Remove button. Since the Remove command cannot be undone a popup
box asks you to confirm this action.
Note: Any spots you have added to the MatchSet Master from the removed gel will
remain in the Master.
5.4 Landmarking Spots
Landmarks are required before you can use Classic Matching. They are also used to
correct errors made by Automated Matching. Landmarks are not required for
Automated Matching.
Before you can begin matching spots in the MatchSet, you must landmark key spots
in all the gels. Landmarks are reference spots used to align and position the gels for
matching.
When choosing spots to landmark, you should look for spots that are well-resolved
and present in all members of the MatchSet (except when creating a Master out of
gels with different pH gradients; see 5.6, Cybergels). Avoid spots in clusters, in case
the spot detection mechanism has misidentified the peaks in the cluster. Unless you
are creating a Master out of gels with different pH gradients, you should landmark
spots in all corners of the gels, as well as the middle of the gels.
To begin landmarking, first magnify a region of the MatchSet gels and identify a well-
resolved spot that appears in all the gels. Study the region under different levels of
magnification to make sure you have identified the same spot in all the gels.