Bio-Rad EXQuest Spot Cutter - Manual (Page 78)

General Operation
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Note: Some commands in PDQuest become mouse-assignable if you are displaying
multiple subwindows. For example, if you select Spots > Spot Crosshairs with a
single image displayed in a window, crosshairs will simply be displayed.
However, if you select Spots > Spot Crosshairs with multiple subwindows
displayed, the command will be assigned to your mouse and you must click a
subwindow to display the crosshairs in that subwindow.