C global actions, 6 sample database, A associating sample to gels – Bio-Rad EXQuest Spot Cutter User Manual

Page 208: Sample database -17

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6.5.c

Global Actions

Global actions are much the same as the adding and removing of individual spots but
are performed on all the spots in the cell.

Auto Add All - allows you to automatically add all the spots of the selected cell to all
the gels. For instance, a replicate group of three gels may contain 15 spots located in
two of the gels. Clicking Auto Add All will add the spots to the gels where they do
not appear.

Auto Cancel All – Cancels all the spots in the cell. This does not remove the spot
from the list, but it does remove it from your analysis.

Auto Remove All – removes the selected spots from the gels.

Since the global actions affects multiple spots on multiple gels, PDQuest warns you
before proceeding with the action.

Performing a global action causes the consensus summary table to automatically
update. There is no need to manually update the Consensus Summary table.

6.6

Sample Database

The Sample database of PDQuest allows you to track gel - sample associations. With
the sample database you can attach attributes to samples. This is important for
tracking two different gels of the same sample type. For instance, your MatchSet may
contain a series of E. coli samples but with different treatments such as temperature.

6.6.a Associating Sample to Gels

To associate samples to gels, select Analyze>Describe Samples>Enter Sample
Attributes.

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