Bio-Rad EXQuest Spot Cutter - Manual (Page 370)

Appendix B. ChemiDoc
B-5
focusing on, you can place a ruler in the ChemiDoc cabinet so that it is visible in
the image.
B.3 Step II. Image Mode
The Image Mode option buttons change the type and scale of the data, as well as the
behavior of the ChemiDoc when acquiring an image.
UV
Select this mode for fluorescent samples. With this mode selected, the data will be
measured in linear intensity units.
White Light
Select this mode for reflective and transmissive samples. With this mode selected, the
data will be measured in uncalibrated optical density (uOD) units.
Chemi
This mode is designed for chemiluminescent samples. With this mode selected, the
data is measured in linear intensity units; however, the data is inverted, so that
samples will appear dark on a light background.
Also, Chemi mode changes the behavior of the Auto and Manual Expose functions, as
described above.
B.4 Step III. Acquire Image
For many white light applications, you can skip this step and save and print images
directly from Live/Focus mode.
For UV light, chemiluminescent applications, or faint samples, the ChemiDoc control
panel has several features for creating image exposures. You can take an automatic