Bio-Rad EXQuest Spot Cutter - Manual (Page 555)

Index-16
PDQuest User Guide
Spot cutter
Basic tool ......................................................................................................... 8-1
Calibration ............................................................................................. 8-6, 9-13
Cut run options ............................................................................................... 8-15
Cutting tip height ................................................................................... 8-3, 9-11
Focussing the camera ............................................................................. 8-3, 9-10
Settings ................................................................................................... 8-4, 9-11
Setup ...................................................................................................... 8-2, 9-10
Wash positions ....................................................................................... 8-6, 9-13
Well positions ........................................................................................ 8-5, 9-13
Spot Detection Wizard ............................................................................................ 4-1
Background subtraction ................................................................................... 4-7
Finding spot centers ......................................................................................... 4-4
Identifying faint, small, and large spots ........................................................... 4-3
Loading images ................................................................................................ 4-2
Noise removal .................................................................................................. 4-7
Parameters, loading .......................................................................................... 4-8
Processing gels ................................................................................................. 4-9
Saving parameters ............................................................................................ 4-8
Sensitivity and size scale ................................................................................. 4-5
Streak removal ................................................................................................. 4-7
Spot Ellipses .......................................................................................................... 4-14
Spot Review Tool .................................................................................................... 6-1
Spots
Adding ........................................................................................................... 4-16
Boundary tools ............................................................................................... 4-18
Cancelling ...................................................................................................... 4-22
Combining ..................................................................................................... 4-23
Contour tool ................................................................................................... 4-19
Crosshairs ....................................................................................................... 4-14
Detecting .......................................................................................................... 4-9
Detection parameters, selecting ....................................................................... 4-1
Detection report ............................................................................................. 4-11
Detection Wizard ............................................................................................. 4-1
Ellipses ........................................................................................................... 4-14
Faint ............................................................................................................... 4-24
Finding ........................................................................................................... 4-28
Freehand tool ................................................................................................. 4-20
Low quality sets ............................................................................................. 4-27