Bio-Rad EXQuest Spot Cutter - Manual (Page 111)

PDQuest User Guide
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Note: If you want to center your arrow on a particular point on the image (e.g., to align
along a particular lane), you can use the F3 key command. Position your cursor
on the point on the image you want to center on, and press the F3 key. The image
will move so that the center of the arrow and your cursor point are aligned.
To complete the rotation, click the Rotate button in the small dialog box. A
subwindow will open containing the rotated image. You will then have the option of
renaming your new image and changing the version number.
If you are not satisfied with your rotated image, simply delete it and start over.
Note: Because an image is composed of square or rectangular pixels, Custom Rotation
has to perform some minor smoothing on the image to turn it at a non-90
o
angle.
In addition, any analysis performed on the image cannot be rotated and will be
lost.
3.15 Filtering Images
Filtering is a process that removes small noise features on an image while leaving
larger features (like data) relatively unaffected. A wide range of filters are available
for removing different types of noise from images. Depending on the nature of your
data, you will probably need to use only one or two of the available filters. However,
you should experiment with several different filters before selecting the ones that
work best for your images.
The filtering commands are located on the Image menu and toolbar.