Bio-Rad EXQuest Spot Cutter - Manual (Page 309)

PDQuest User Guide
9-20
Step 3: Perform alignment.
In Step 3, you will have the option of skipping auto-alignment by making the software
work in totally manual mode. This could be useful if you have very few spots to be
cut, so you can do the alignment yourself.
Step 4: Set the confidence threshold value. Visually confirm and, if needed, manually
correct the alignment of all those spots whose alignment is below the confidence
threshold.
Fig. 9-9. Aligning the images.
In the Align Images dialog, the left window displays the MatchSet image, and the
right window will contain the cutter camera image of the corresponding gel.