Bio-Rad EXQuest Spot Cutter User Manual

Page 281

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PDQuest User Guide

8-16

Fig. 8-11. Cuts being made.

Confirming Cuts

For visible spots, inspect the gel to confirm that each cut is being made correctly.

For fluorescent spots, you must take a picture of the gel using UV light to ensure that
cuts are being made correctly. After one or more cuts have been made, click Pause to
pause the cut run. Note that the cutter will pause only after the most recent cut has
been deposited in a well.

Confirm the pause, then click the Confirm Cuts button under the main acquisition
window.

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