Bio-Rad EXQuest Spot Cutter - Manual (Page 125)

PDQuest User Guide
4-4
Fig. 4-3. Image with large, small, and faint spots defined.
4.1.b Step 2. Test Settings
In this step, you first see how many spots are detected based on the settings in Step 1,
then you adjust those settings until all significant spots are identified.
Note: Before proceeding, if there are streaks in your image, select the Vertical and/or
Horizontal streak removal checkboxes under Optional Controls.
Click the Find Spot Centers button. Noise smoothing, background
subtraction, and (if selected) streak removal will be performed,
and the spot centers will appear marked with crosshairs.
Review the results of spot detection by studying the crosshairs on the image. The
number of spots detected is displayed in the Spot Count field.