Bio-Rad EXQuest Spot Cutter - Manual (Page 306)

Integrated Excision Tool
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Step 1. Select the Cut List
At the top of the Integrated Excision Tool dialog, click the pulldown button to select
from the available cut lists you created using the Excision Gel Selection tool.
The cut list will be displayed in table format in the middle of the dialog box. The cut
list lists the microplate well, spot number, gel, spot quantity in that gel, cut run
number and number of cuts for each spot.
The cuts are listed in the table in order of gel, so that all the cuts will be performed on
one gel before you are prompted to load the next one.
At the beginning of each row is a Cut checkbox, specifying the spots that will be cut
in the current cut run. Uncheck the box if you do not want to perform that cut.
After each cut is performed, its status will be listed as “completed” and its cut
checkbox will be deselected.
After a cut run is complete, you can reselect checkboxes in the list to recut any missed
spots or cut spots that were skipped in the previous run.
Step 2. Select the Cut Run Options
Before beginning a cut run using the Integrated Excision Tool, you must select the
appropriate cutter options for that run.
Select Spin When Cutting to make the cutting tip spin as it cuts.
Spin When Ejecting will cause the cutting tip to spin as it ejects the cut into a
microplate well.
Cut Longer will cause the cutting tip to hold in the down position longer when
making a cut.
Double Eject will cause the ejected pin in the cutting tip to eject twice into a
microtiter plate well.
The Gel Defaults button automatically selects the most useful options when cutting
gels. The PVDF Defaults button automatically selects the most useful options when
cutting PVDF membranes.