Bio-Rad EXQuest Spot Cutter - Manual (Page 225)

PDQuest User Guide
6-34
Display All SSPs displays SSP numbers next to every spot in every open window.
Transferring SSP Numbers in a higher level MatchSet
Transfer SSPs is used to copy the SSP numbers from one higher level MatchSet
member to the others. This makes it easier to correlate data from the member
MatchSets.
With the higher level MatchSet open, select Transfer SSPs from the submenu. In the
first pop-up box, select the MatchSet member you want to copy from; in the second
box, select the member you want to copy to. You will be asked to confirm the transfer.
When the transfer is complete, the number of SSP numbers transferred will be listed
in an alert box. Repeat the procedure to apply the numbers to all the members in the
MatchSet.
6.10 Database Spot Numbers
Database spot numbers (DSNs) are an alternative to the SSP numbers. The DSN
system provides a more flexible way to number protein spots in a Master in that it
indicates the general location of each spot. A coordinate grid can be established for
each axis, based on molecular weight and isoelectric point. The grid coordinates are
identified with letters (A–R, excluding I and O because they look like numbers). The
letters are intended to represent specific MrpI ranges so that the numbering between
different gels can be comparable. These ranges are as follows:
Mr values: 0-10=A 87-117=J
11-14=B 118-158=K
15-19=C 159-215=L
20-25=D 216-293=M
26-34=E 294-398=N
35-46=F 399-541=P
Mr values (cont): 47-63=G 542-736=Q
64-86=H 737-1000=R
pI values: 0-2=A 7.0-7.5=J
2-3=B 7.5-8.0=K