Bio-Rad EXQuest Spot Cutter - Manual (Page 526)

L-1
Appendix L
Calibration and Merging
You can use calibration strips (calstrips) to calibrate your scans for more accurate
quantitation. Calstrips are required for both gel calibration and merging multiple
exposures.
L.1 Calibrating Gels
The commands for calibrating gels are located on the Edit > Calibrate Gels submenu.
Fig. L-1. Edit > Calibrate Gels submenu.
Creating a Calstrip File
If calstrip images are currently part of your gel images, you will have to crop them
and save them as separate files for use in PDQuest. To do so, select Advanced Crop >
Define Calstrip Area from the Image menu, draw a box around your calstrip, then
crop it and save it as a calstrip file. See section 3.13.a for instructions.