Bio-Rad EXQuest Spot Cutter - Manual (Page 516)

Appendix K. VersaDoc
K-9
The following table provides recommended exposure times for various applications
Table 1: Recommended Exposure Times and Lenses
Sample
Recommended
Exposure Lens & Filter Accessories Used
Fluorescent Stain Gel 3–30 sec. Zoom/IR None
Fluorescence End-
Label Gel 30 sec.–5 min. Zoom/IR None
Fluorescent Blot 0.5–5 sec. Zoom/IR Sample/Chemi
Tray (if sample is
small)
Chemifluorescent
Blot 0.5–5 sec. Zoom/IR None
Colorimetric Gel 0.1–1 sec. Zoom/IR White light con-
version screen
Colorimetric Blot 0.1–1 sec. Zoom/IR Sample/Chemi
Tray (if sample is
small)
X-ray film 0.1–1 sec. Zoom/IR White light con-
version screen
Weak
Chemiluminescence
1
5–10 min. 50 mm Sample/Chemi
Tray
(if sample is
small)
Strong
Chemiluminescence
1
10 sec.–2 min. 50 mm Sample/Chemi
Tray
(if sample is
small)
1
For chemi applications, the 50mm lens is recommended. Always remove the 660 fil-
ter.