Bio-Rad EXQuest Spot Cutter - Manual (Page 181)

PDQuest User Guide
5-30
Fig. 5-17. Edit matches submenu commands.
Manual Matching
After you have landmarked numerous spots and reviewed the results of auto-
matching, you are ready to identify the significant spots that could not be auto-
matched. To highlight these spots in a member gel, use the Mark Unmatched Spots
command (Shift+F8, Match > Edit Matches submenu) in the gel’s subwindow.
Unmatched spots are marked with red circles. Use the Zoom Box tool to study each
unmatched spot in detail in each gel.
If a spot was missed during spot detection, you must add it to the Gaussian image,
then match. If a spot was not included in the Master, you must add it to the Master,
then match. If an unmatched spot is not significant, you can cancel it.