Bio-Rad EXQuest Spot Cutter - Manual (Page 313)

PDQuest User Guide
9-24
camera image to the correct position. Note that you can in fact drag any spot on the
camera image any time to manually correct its alignment. Repeat the above procedure
for every spot to be cut in this gel.
Fig. 9-13. Matching spots above and below confidence level.
Note: Note that the green circle represents the actual cut made by the cutting tip in the
gel, and will vary in size depending on the cutting tip size selected under Spot
Cutter Settings.
The following display options are available in the Align Analysis and Cutter Images
dialog box:
Cut Marks will mark the spots to be cut with red Xs in the analysis image.
Matches will mark matched spots with green circles in both the MatchSet image and
cutter camera image.
Auto-zoom will automatically change the magnification and position of the (right-
hand) camera cutter image to imitate the display in the analysis image.
Matched
Spots to be
matched
spots
Spot displayed
in step 4