Bio-Rad EXQuest Spot Cutter - Manual (Page 169)

PDQuest User Guide
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algorithm PDQuest has used in previous versions. If you select this method, the
primary phase can be thought of as "auto-landmarking".
Extended matches are shown in blue.
Getting started
First you must create a MatchSet and designate a Master image. Once this is done,
select Match > Automated Matching.
If you simply want to match all the spots using the default settings, press the Match
All button, then the Done button.
If you want to explore the match settting, select a member using the Members pull-
down button. Then use the sliders to change the settings and press the Match button.
You will see the number of primary matches and the number of extended matches on
the dialog.
You will probably want to configure the MatchSet window to display only the master
and the member you are matching. You can do this with theWindow> Configure Sub-
windows command. Inspect the matches for accuracy and completeness.
Once you have obtained the best settings for your images, press Match All to apply
those settings to all members and match all members. You will be prompted for a
name for the settings. When matching subsequent images, you can recall these
settings by name.
Since the matching models are difficult to describe adequately in words, we
recommend that you explore the various matching methods on your images and find a
combination that gives the best results: the most correct matches with a minimal
number of incorrect matches. The incorrect matches are easier to find using the spot
review tool than are missing matches.
Classic Matching
By default, Automated Matching occurs immediately after each landmark is placed in
all the MatchSet members. To turn off this feature, deselect Auto-match after
Landmark in the Applications tab under Edit > Preferences.