Bio-Rad EXQuest Spot Cutter - Manual (Page 124)

Detecting and Editing Spots
4-3
Fig. 4-2. Selecting a new image in the Spot Detection Wizard.
The control panel in the Wizard is designed to guide you through the parameter
selection process:
4.1.a Step 1. Give Manual Guidance
In this step, you mark features on the gel scan that will be used to automatically select
the correct spot detection parameters.
1.Click the Faint Spot button, then click on the faintest spot in the scan
that you want to detect. This will set the sensitivity and minimum peak
value parameters.
2.If the smallest spot you want to detect is smaller than the faintest
spot, click the Small Spot button, then click on the smallest spot. This
will set the size scale parameter.
3.Click the Large Spot button, then draw a box around the largest spot
on the image that you want to detect. This sets the radius of the
background subtraction rolling ball and the streak removal rolling
disk.