A review individual spots – Bio-Rad EXQuest Spot Cutter User Manual

Page 206

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Analysis Tools

6-15

column lists the number of spots that appear on one gel and can be matched to more
than one spot on one or more of the other gels.

Fig. 6-12. Consensus Summary Table

Use the Cancel, Review, and Accept buttons to organize the different Consensus
Summary cells according to your review criteria. Marking a cell Cancel will color
code it red and remove it from automatic inclusion in the list of spots to be reviewed
with the Individual Spot Tools later. Similarly, marking a cell Accept will color code
it green and remove it from automatic inclusion in the review list. Note that any cell,
even those marked Cancel or Accept, can be manually selected for review later

Click Update Consensus to update the Consensus Summary table with any manual
changes made in the Review tool. Rematch will rematch all the spots in the replicate
group.

Note:

If you rematch the gels in a replicate group, you will lose all prior matching.

The Global actions and Review tool sections of the Group Review affect the cell you
have selected in the Consensus Summary table (highlighted in blue). The global
Actions affects all the spots in the selected cell, while the Individual Review tool
allows you to review each spot individually and add or remove the spot (represented
by a blue triangle in the gel images).

6.5.a Review Individual Spots

The Review Individual Spots section of the Group Consensus allows you to
individually review each spot in the group. The active group field shows the spot
number of the highlighted cell in the Consensus Summary table. Use the arrows to

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