Bio-Rad EXQuest Spot Cutter - Manual (Page 17)

PDQuest User Guide
1-4
1.3 PDQuest Workflow
Fig. 1-2. Steps involved in using PDQuest.
Image Acquisition
PDQuest can acquire images of gels using Bio-Rad’s densitometers, storage phosphor
imagers, and camera-based imaging systems.
First you open the acquisition window for your imaging device, and capture the
image using the controls. Gel images are saved on your hard disk, network file server,
or removable storage media. The displayed image in PDQuest is ready for analysis.
Image Sizing and Orientation
In this step, you adjust the size and orientation of the image by using the cropping and
rotating tools on the Image menu.
Acquire the image
Size and orient the image
Spot identification
Spot comparison and
matching
Data analysis
Spot cutting and mass spec
analysis
Publish results