Bio-Rad EXQuest Spot Cutter - Manual (Page 303)

PDQuest User Guide
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Note: If you are using the lens filter for UV light, make sure that it is installed BEFORE
calibration. If you add or remove the lens filter after calibration, you must
recalibrate.
Click the Calibrate Cutter button. A series of dialog boxes will guide you
through the calibration procedure. The first dialog box will prompt you to
specify whether you have installed the lens filter or not. The lens filter must
be INSTALLED if you are using the fluorescent spot cutter in fluorescent mode. The
lens filter is not required if you are using the spot cutter only in visible light mode.
Step 1: The second dialog box prompt for flat field calibration requires that you ensure
the stage is completely empty and dry. This includes the cutting mat, which must
be removed. Press Ready to proceed. If you have previously performed this step
you can skip to Step 2, although this is not recommended.
Fig. 9-6. Spot cutter calibration step 2.
Step 2: The third dialog requires the calibration template grid pattern supplied with the
PDQuest system. Place the grid on top of the cutting mat, which should have