Bio-Rad EXQuest Spot Cutter - Manual (Page 220)

Analysis Tools
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6.8 MrpI Data
In a MatchSet, you can enter the molecular weight and isoelectric point values for
your known protein spots. With this data, PDQuest can calculate MrpI values for all
the spots in the MatchSet.
The functions needed to enter and display MrpI data are found in the Analyze > MrpI
Standards submenu and the MrpI Tools toolbar.
Fig. 6-21. MrpI commands.
Note: PDQuest needs to know the pH gradient of the gel in order to calculate isoelectric
point values. To enter the correct orientation, go to Edit > Preferences >
Applications tab and click either Acid-Base or Base-Acid next to the pH Gradient
prompt.