Bio-Rad EXQuest Spot Cutter - Manual (Page 194)

Analysis Tools
6-3
At the top of the review tool window, select All Spots to display histograms of all the
spots in the MatchSet or Analysis Set to display histograms of only the spots in a
specified set. If you select the analysis set option, a pop-up list of available analysis
sets will be displayed. Select the set you want to display from the list.
You can reorder the graphs by clicking the Sort By buttons. You can sort by Standard
Spots SSP number (default) or Database Spot (DSN) (if defined). If you select Trend,
the graphs are arranged based on a linear regression model of spot presence and
quantity in each member. If you select Quantity, the graphs are arranged starting with
spots of greatest quantity to spots of least quantity.
To scroll through the pages of histograms, click First, Prev, Next, or Last with the red
arrows above the graphs.
If you click on a histogram of a spot, that spot will be magnified and highlighted in all
the MatchSet gels. You can then detect and match the spot using the other tools in
PDQuest.
If you click on a histogram, the histogram itself will be highlighted by a blue
background, indicating that it is selected. You can select more than one histogram at a
time. To deselect a histogram, click on it again.
Note: To highlight and magnify spots in the MatchSet without selecting the histograms,
shift-click each histogram.