5 . matchsets, Matchsets -1, Matchsets – Bio-Rad EXQuest Spot Cutter User Manual

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5-1

5. MatchSets

After the protein spots in your gels have been detected, you are ready to compare
spots across gels. To do so, you must create a MatchSet. You can create a MatchSet in
two different ways, with the Match > New MatchSet command, or through the Spot >
Automated Detection and Matching dialog, Step 3. The commands for editing
MatchSets are located in Match > MatchSet Tool and Match >Edit Matches submenu.

A MatchSet is PDQuest’s mechanism for comparing and analyzing the spots in an
experiment. It contains copies of the Gaussian and Filtered images of the gel(s) in an
experiment.

Fig. 5-1. How experiments are organized into MatchSets.

Note:

If the Copy option is specified when creating the MatchSet, see section 5.1.b,
Member Sharing.

Gel 1
Image

Gel 2
Image

Gel 3
Image

Gel 1
Copy

Gel 2
Copy

Gel 3
Copy

Master

Experiment

MatchSet

Master

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