Bio-Rad EXQuest Spot Cutter - Manual (Page 300)

Integrated Excision Tool
9-11
Manually adjust the camera lens and position as described in the hardware manual to
optimize the image. When you are satisfied with the image and the levels of gray
scale, click Stop to close the Focus dialog.
Set Cutting Tip Height
Click the Set Cutting Tip Height button to adjust the distance the cutting tip
drops down to cut a spot. The adjustment dialog box will open, and the
cutting tip will move to the center of the gel.
Click Lower Tip to drop the cutting tip to its lowest position. In this position,
manually adjust the cutting tip height as described in the hardware manual until it
presses lightly against the surface of the cutting mat.
Raise Tip raises the cutting tip. Test Cut performs a test cut in the center of the gel.
Click Done to accept the settings and return the cutting tip to home position.
The cutting tip height only needs to be set once, unless the spot cutter gets out of
alignment.
Spot Cutter Settings
Click the Spot Cutter Settings button to open the settings dialog box for the
cutter.