Bio-Rad EXQuest Spot Cutter - Manual (Page 244)

Analysis Sets and Annotations
7-17
All Spots in Member
The All spots in member option will create an analysis set containing all the spots in a
selected member that are matched to the Master. Click the drop down button to the
right of the member field to display a list of the members from which to choose. The
member you chose displays in the Member field. The analysis set will include all the
spots in the member you selected that are matched to the Master.
Saturated Spots
Saturated spots will create an analysis set of all spots that are saturated in any
member.
Importing Spots from External Analysis
PDQuest allows you import a set of spots from another MatchSet or a spot list
exported from some other analysis application.
Standard Spot (SSP) numbers are unique numbers that are automatically assigned to
each spot in the MatchSet standard when you create the standard. Selecting Import
list of SSPs from text file option opens the Select file to import dialog. Navigate to the
directory where the Standard Spot Numbers text file is located.
As with all other types of analysis sets, when the Analysis Set Template is open, you
can view the results in the MatchSet immediately. To the right of the dialog, you can
specify the spot marker, on which gel(s) to show the spots, and what information to
show on the gel(s).
When you open the Analysis set Template, whatever analysis sets you have selected
in the ASM become inactive. To view these sets in the Analysis set Template, check
the box labeled, “Display Selected sets.”
7.1.g Boolean Analysis Sets
Boolean analysis sets are formed by combining two or more previously defined
analysis sets using Boolean operators. You can create Boolean sets that include:
1. The intersection of two or more sets of spots (proteins present in all sets).
2. The union of two or more analysis sets (all spots in all sets).