Bio-Rad EXQuest Spot Cutter - Manual (Page 126)

Detecting and Editing Spots
4-5
Fig. 4-4. Spot crosshairs marked in the Spot Detection Wizard.
If all the spots you want to identify have been detected, you can save this parameter
set. If significant spots have been missed, you can change the faint, small, and large
spot selections in Step 1, or change the Sensitivity, Min Peak, and Size Scale directly.
Note: Adjustments to these settings will automatically redetect spots.
Sensitivity
If significant faint spots in the scan have been missed, you can use the Faint Spot tool
in Step 1 to change your faint spot selection, or you can increase the Sensitivity
setting by typing a number directly in the field (next to the Find Spot Centers button)
or clicking on the adjustment arrows. Higher sensitivity will detect more spots. If
image noise has been detected as spots, decrease the sensitivity or perform additional
speckle removal.